Roja entered the cinema industry with Telugu films. Her first movie was Prema Thapassu. She was introduced to the Tamil film industry by director R.K. Selvamani with Chembaruthi along with actor Prashanth. The movie was a hit and paved way for role in another success, Suriyan with Sarath Kumar. Both the films established her in Tamil cinema. Her performance in films with actors such as Rajnikanth in Veera, and Arjun Sarja in Ayudha Poojai were praised. Roja's major career breakthrough was in the film Unnidathil Ennai Koduthen, directed by Vikraman. Her 100th movie was Pottu Amman. Roja later appeared in supporting roles in films like Arasu (2003), Parijatham (2006), Shambo Shiva Shambo (2010), Golimar (2010), Mogudu (2011), Kodipunju (2011), Veera (2011), Kaavalan (2011) and Saguni (2012).